proteome profiler human phospho kinase antibody array  (R&D Systems)

Journal: bioRxiv

Article Title: Investigating the impact of fibroblast proximity to a tumor on fibroblast extracellular vesicle production utilizing 3D bioprinted stromal models

doi: 10.1101/2024.12.02.626455

Figure Lengend Snippet: NTAF media has increase in cancer progression related cytokines. A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.

Article Snippet: Cytokine profiling of the EV groups was performed using the dot blot-based Proteome Profiler Human XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions.

Techniques:

Journal: Journal of Cosmetic Dermatology

Article Title: New Plant Extracts Exert Complementary Anti‐Hair Loss Properties in Human In Vitro and Ex Vivo Models

doi: 10.1111/jocd.16616

Figure Lengend Snippet: Phosphorylation of receptor tyrosine kinases (RTKs) and their downstream effectors in human follicle dermal papilla cells treated with a Silybum marianum extract (SME, 30 μg/mL, dissolved in dimethylsulfoxide [DMSO]) or DMSO for 1 h ( n = 2 donors, tests performed in duplicate for DMSO‐treated and SME‐treated cells from each donor). (a) EGFR and PDGFRβ, and (b) the downstream phosphokinases ERK1/2, GSK3α/β, Akt1/2/3, STAT5α and STAT5α/β. Data were obtained using the Human Proteome Profiler Phospho‐RTK and Phospho‐Kinase array kits, respectively. Each bar represents the mean ± SEM of SME/DMSO ratio.

Article Snippet: Growth factor receptor (GFR) signaling pathways were analyzed using two Human Proteome Profiler antibody array kits (R&D Systems, USA) to simultaneously detect the phosphorylation of 49 receptor tyrosine kinases (Proteome Profiler Human Phospho‐RTK Array Kit) and of 43 kinases and two related total proteins (Proteome Profiler Human Phospho‐Kinase Array Kit).

Techniques:

Journal: Experimental & Molecular Medicine

Article Title: Sorcin can trigger pancreatic cancer-associated new-onset diabetes through the secretion of inflammatory cytokines such as serpin E1 and CCL5

doi: 10.1038/s12276-024-01346-4

Figure Lengend Snippet: a Human cytokine array of supernatants from PANC-1 cells treated with CM- NC -siRNA or CM- SRI -siRNA. b Quantitative analysis of downregulated components in PANC-1 cells treated with SRI siRNA in ( a ). c Detection of the mRNA levels of SRI and 5 downregulated cytokines by RT‒PCR in PANC-1 cells. Fresh culture media containing inflammatory factors was changed every 12 h until 72 h after MIN6 cells were seeded, and the insulin content in the supernatant after GSIS in MIN6 cells was treated with gradient concentrations of ( d ) CCL5 and ( e ) serpin E1. The phosphorylation levels of p38 in MIN6 cells incubated with different concentrations of ( f ) CCL5 and ( g ) serpin E1 at concentrations of 0, 25, 50 and 100 nM. h Detection of the phosphorylation level of the p38 pathway in MIN6 cells treated with different conditioned media from PANC-1, CFPAC-1 and AsPC-1 cells for 72 h. i Photos of nude mice following subcutaneous injections of pCDH- NC or pCDH- SRI PC cells and subsequent intratumoral injections of PBS, CCL5, or serpin E1 after tumor formation. j Fasting blood glucose detection in nude mice after absolute fasting for 24 h before sacrifice. k Fasting insulin level detection in nude mice after absolute fasting for 24 h before sacrifice. l Photos of subcutaneous tumors after surgical removal. m Quantitative results of tumor volume. n Immunofluorescence of islets in the pancreas of nude mice labeled with insulin and PDX1 proteins. Ns, not significant; * P < 0.05; ** P < 0.01, means ± SD are shown. Statistical analysis was performed via Student’s t test for two groups.

Article Snippet: A membrane-based antibody array (Proteome Profiler Human Cytokine Array Kit, R&D Systems, ARY005B) was used to profile 36 soluble proteins, mostly cytokines and chemokines, in the conditioned medium from PANC-1 cells transfected with either SRI -siRNA or NC-siRNA.

Techniques: Incubation, Immunofluorescence, Labeling